The Tokyo-based firm has been awarded a payment institution (PI) licence for the European Union, building on its regulatory approval in the US and Japan.
BitFlyer handles about a quarter of the globe's bitcoin exchange volumes, with more than $250bn (£180bn) worth of bitcoin being traded on its platform last year.
Yuzo Kano, founder and chief executive of bitFlyer, said: “When I set up bitFlyer in 2014, I did so with global ambitions and the belief that approved regulatory status is fundamental to the long-term future of bitcoin and the virtual currency industry.
“I am proud that we are now the most compliant virtual currency exchange in the world; this coveted regulatory status gives our customers, our company and the virtual currency industry as a whole a very positive future outlook.”
BitFlyer is the only exchange licensed in Europe, and can give traders access to the globe's biggest bitcoin trading market in Japan.