These fans have humidistat and temp control, we are going to run the controlls for every fan down to where the switches for powering on and off each fan are so it is easier to access
Definitely screw them into the boards they're being mounted to.
Also, the humidity and temp SENSORS are in those boxes with the controls, are you sure you want to move them away from where the fans are at?
Kind of a tradeoff if you do or if you don't, depending on where you DO mount them - right at the intake area for your miner shelves should work well.
Gonna need more conduit though if you do move them, for core requirements - or some way to "hide" the wiring in the wall(s).
A single 2x4 in the middle of the hole isn't going to affect airflow noticeably, shouldn't be an issue.
Might have made sense to put a centering hole in from underneath FIRST though to avoid that.